Abstract
Two-dimensional polyacrylamide gel electrophoresis (2DE) and Western immunoblotting have proven to be invaluable and fundamental techniques for comprehensive characterization of food allergens. Here, we describe and discuss detailed protocols used in our studies on identification of allergenic proteins in shrimp, sesame, hazelnut, and pistachio.
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Grishina, G., Bardina, L., Grishin, A. (2017). 2D-Electrophoresis and Immunoblotting in Food Allergy. In: Lin, J., Alcocer, M. (eds) Food Allergens. Methods in Molecular Biology, vol 1592. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6925-8_5
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DOI: https://doi.org/10.1007/978-1-4939-6925-8_5
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