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Biosensors and Biodetection pp 71–88Cite as

Microfluidic Arrayed Lab-On-A-Chip for Electrochemical Capacitive Detection of DNA Hybridization Events

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1572))

Abstract

A microfluidic electrochemical lab-on-a-chip (LOC) device for DNA hybridization detection has been developed. The device comprises a 3 × 3 array of microelectrodes integrated with a dual layer microfluidic valved manipulation system that provides controlled and automated capabilities for high throughput analysis of microliter volume samples. The surface of the microelectrodes is functionalized with single-stranded DNA (ssDNA) probes which enable specific detection of complementary ssDNA targets. These targets are detected by a capacitive technique which measures dielectric variation at the microelectrode–electrolyte interface due to DNA hybridization events. A quantitative analysis of the hybridization events is carried out based on a sensing modeling that includes detailed analysis of energy storage and dissipation components. By calculating these components during hybridization events the device is able to demonstrate specific and dose response sensing characteristics. The developed microfluidic LOC for DNA hybridization detection offers a technology for real-time and label-free assessment of genetic markers outside of laboratory settings, such as at the point-of-care or in-field environmental monitoring.

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Acknowledgments

The authors would like to thank the Robert W. Deutsch Foundation for financial support. The authors also appreciate the support of the Maryland NanoCenter and its FabLab.

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Authors and Affiliations

  1. Department of Biomedical Engineering, Ben-Gurion University of the Negev, Beer Sheva, 8410501, Israel

    Hadar Ben-Yoav

  2. MEMS Sensors and Actuators Laboratory (MSAL), Department of Electrical and Computer Engineering, Institute for Systems Research, University of Maryland, College Park, MD, 20742, USA

    Peter H. Dykstra & Reza Ghodssi

  3. Fischell Department of Bioengineering, University of Maryland, College Park, MD, 20742, USA

    Peter H. Dykstra, William E. Bentley & Reza Ghodssi

Authors
  1. Hadar Ben-Yoav
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  2. Peter H. Dykstra
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  3. William E. Bentley
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  4. Reza Ghodssi
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Corresponding author

Correspondence to Hadar Ben-Yoav .

Editor information

Editors and Affiliations

  1. National Cancer Institute, National Institutes of Health, Rockville, Maryland, USA

    Ben Prickril

  2. National Cancer Institute, National Institutes of Health, Rockville, Maryland, USA

    Avraham Rasooly

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Ben-Yoav, H., Dykstra, P.H., Bentley, W.E., Ghodssi, R. (2017). Microfluidic Arrayed Lab-On-A-Chip for Electrochemical Capacitive Detection of DNA Hybridization Events. In: Prickril, B., Rasooly, A. (eds) Biosensors and Biodetection. Methods in Molecular Biology, vol 1572. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6911-1_6

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  • DOI: https://doi.org/10.1007/978-1-4939-6911-1_6

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6910-4

  • Online ISBN: 978-1-4939-6911-1

  • eBook Packages: Springer Protocols

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