Abstract
The bimodal waveguide (BiMW) sensor is a novel common path interferometric transducer based on the evanescent field detection principle, which in combination with a bio-recognition element allows the direct detection of biomolecular interactions in a label-free scheme. Due to its inherent high sensitivity it has great potential to become a powerful analytical tool for monitoring substances of interest in areas such as environmental control, medical diagnostics and food safety, among others. The BiMW sensor is fabricated using standard silicon-based technology allowing cost-effective production, and meeting the requirements of portability and disposability necessary for implementation in a point-of-care (POC) setting.
In this chapter we describe the design and fabrication of the BiMW transducer, as well as its application for bio-sensing purposes. We show as an example the biosensor capabilities two different applications: (1) the immunodetection of Irgarol 1051 biocide useful in the environmental field, and (2) the detection of human growth hormone as used in clinical diagnostics. The detection is performed in real time by monitoring changes in the intensity pattern of light exiting the BiMW transducer resulting from antigen–antibody interactions on the surface of the sensor.
*These authors contributed equally to this work.
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Acknowledgments
The nanoB2A is a consolidated research group (Grup de Recerca) of the Generalitat de Catalunya and has support from the Departament d’Universitats, Recerca i Societat de la Informació de la Generalitat de Catalunya (2014 SGR 624). ICN2 is the recipient of Grant SEV-2013-0295 from the “Severo Ochoa Centers of Excellence” Program of Spanish MINECO. The authors acknowledge to the European Union (BRAAVOO Grant Agreement No 614010). The authors thank Dr. Parlow (NIDDK, CA, USA), Dr. Rodríguez-Frade and Dr. M. Mellado (CNB-CSIC, Madrid, Spain), and Prof. Marco (IQAC-CSIC, Barcelona, Spain) for the supply of the inmunoreagents.
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Herranz, S., Gavela, A.F., Lechuga, L.M. (2017). Label-Free Biosensors Based on Bimodal Waveguide (BiMW) Interferometers. In: Rasooly, A., Prickril, B. (eds) Biosensors and Biodetection. Methods in Molecular Biology, vol 1571. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6848-0_11
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DOI: https://doi.org/10.1007/978-1-4939-6848-0_11
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