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Biosensors and Biodetection pp 143–160Cite as

Resonant Waveguide Grating Imager for Single Cell Monitoring of the Invasion of 3D Speheroid Cancer Cells Through Matrigel

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1571))

Abstract

The invasion of cancer cells through their surrounding extracellular matrices is the first critical step to metastasis, a devastating event to cancer patients. However, in vitro cancer cell invasion is mostly studied using two-dimensional (2D) models. Three-dimensional (3D) multicellular spheroids may offer an advantageous cell model for cancer research and oncology drug discovery. This chapter describes a label-free, real-time, and single-cell approach to quantify the invasion of 3D spheroid colon cancer cells through Matrigel using a spatially resolved resonant waveguide grating imager.

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Author information

Authors and Affiliations

  1. Biochemical Technologies, Corning Research and Development Corporation, Corning Incorporated, Sullivan Park, Corning, NY, 14831, USA

    Nicole K. Febles, Siddarth Chandrasekaran & Ye Fang Ph.D.

  2. NanoScience Technology Center, Department of Mechanical, Materials and Aerospace Engineering, University of Central Florida, Orlando, FL, USA

    Nicole K. Febles

  3. Department of Biomedical Engineering, Cornell University, Ithaca, NY, USA

    Siddarth Chandrasekaran

Authors
  1. Nicole K. Febles
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  2. Siddarth Chandrasekaran
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  3. Ye Fang Ph.D.
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Corresponding author

Correspondence to Ye Fang Ph.D. .

Editor information

Editors and Affiliations

  1. National Cancer Institute, National Institutes of Health, Rockville, Maryland, USA

    Avraham Rasooly

  2. National Cancer Institute, National Institutes of Health, Rockville, Maryland, USA

    Ben Prickril

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Febles, N.K., Chandrasekaran, S., Fang, Y. (2017). Resonant Waveguide Grating Imager for Single Cell Monitoring of the Invasion of 3D Speheroid Cancer Cells Through Matrigel. In: Rasooly, A., Prickril, B. (eds) Biosensors and Biodetection. Methods in Molecular Biology, vol 1571. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6848-0_10

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  • DOI: https://doi.org/10.1007/978-1-4939-6848-0_10

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  • Online ISBN: 978-1-4939-6848-0

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