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Thermogenic Fat pp 9–16Cite as

Isolation of Mouse Stromal Vascular Cells for Monolayer Culture

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1566))

Abstract

Positive energy balance contributes to adipose tissue expansion and dysfunction, which accounts largely for obesity and related metabolic disorders. Thermogenic fat can dissipate energy, activation or induction of which may promote energy balance and address the pressing health issues. Recent studies have shown that stromal vascular fraction (SVF) from white adipose tissue (WAT) can develop both white and brown-like adipocyte phenotypes, thus serving as a unique model to study adipogenesis and thermogenesis. Here, we describe a protocol for effective isolation of mouse SVF from WAT, induction of differentiation, and detection of adipogenesis. Success tips for isolation and culture of SVF are also discussed.

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Acknowledgment

This work was supported in part by USDA National Institute of Food and Agriculture Hatch Project 1007334 (Z.C.).

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Authors and Affiliations

  1. Department of Human Nutrition, Foods and Exercise, Fralin Life Science Institute, College of Agriculture and Life Science, Virginia Tech, 1981 Kraft Drive, Blacksburg, VA, 24061, USA

    Longhua Liu, Louise D. Zheng, Sarah R. Donnelly & Zhiyong Cheng

  2. Department of Molecular and Integrative Physiology, Life Sciences Institute, University of Michigan, Ann Arbor, MI, USA

    Margo P. Emont & Jun Wu

Authors
  1. Longhua Liu
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  2. Louise D. Zheng
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  3. Sarah R. Donnelly
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  4. Margo P. Emont
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  5. Jun Wu
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  6. Zhiyong Cheng
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Corresponding author

Correspondence to Zhiyong Cheng .

Editor information

Editors and Affiliations

  1. Department of Molecular and Integrative Physiology, Life Sciences Institute, University of Michigan, Ann Arbor, Michigan, USA

    Jun Wu

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Liu, L., Zheng, L.D., Donnelly, S.R., Emont, M.P., Wu, J., Cheng, Z. (2017). Isolation of Mouse Stromal Vascular Cells for Monolayer Culture. In: Wu, J. (eds) Thermogenic Fat. Methods in Molecular Biology, vol 1566. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6820-6_2

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  • DOI: https://doi.org/10.1007/978-1-4939-6820-6_2

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6819-0

  • Online ISBN: 978-1-4939-6820-6

  • eBook Packages: Springer Protocols

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