Abstract
Contact hypersensitivity (CHS) is the most basic murine model for type IV hypersensitivity. This dermatitis model is mediated by hapten-sensitized, skin-infiltrating T cells. Recent intravital imaging studies have demonstrated the dynamic migratory property of skin-infiltrating T cells and the orchestrated interaction of T cells with dermal dendritic cells. Multiphoton microscopy enables the direct, three-dimensional, and minimally invasive imaging of in vivo skin samples with high spatiotemporal resolution. Here, we describe a basic method for the intravital imaging of skin-infiltrating T cells in the DNFB-induced CHS response.
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Acknowledgment
This work was supported by Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan.
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Egawa, G., Kabashima, K. (2017). Visualization of the T Cell Response in Contact Hypersensitivity. In: Clausen, B., Laman, J. (eds) Inflammation. Methods in Molecular Biology, vol 1559. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6786-5_4
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DOI: https://doi.org/10.1007/978-1-4939-6786-5_4
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