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SH2 Domains

Volume 1555 of the series Methods in Molecular Biology pp 77-81

Date:

SH2 Ligand Prediction–Guidance for In-Silico Screening

  • Shawn S. C. LiAffiliated withDepartment of Biochemistry, Schulich School of Medicine and Dentistry, Western UniversityChildren’s Health Research Institute Email author 
  • , Lei LiAffiliated withDepartment of Biochemistry, Schulich School of Medicine and Dentistry, Western UniversityMedical College of Qingdao University

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Abstract

Systematic identification of binding partners for SH2 domains is important for understanding the biological function of the corresponding SH2 domain-containing proteins. Here, we describe two different web-accessible computer programs, SMALI and DomPep, for predicting binding ligands for SH2 domains. The former was developed using a Scoring Matrix method and the latter based on the Support Vector Machine model.

Key words

SH2 SH2-peptide interactions Ligand prediction SMALI DomPep