Abstract
Here, we describe how peptide nucleic acid (PNA) probes can be used to enrich genomic DNA fractions to facilitate downstream analysis, such as the haplotype phasing of the isolated genomic pieces. This method enriches for polymorphic regions of fragmented chromosomes by physically separating the desired sequence and flanking regions. The PNA probes used for enrichment are novel synthetic nucleic acids with highly specific targeting and hybridization properties. Using a enrichment technique, we capture high molecular weight genomic DNA using nothing more than a simple modification to standard genomic DNA extraction from blood.
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Acknowledgment
N.M.M. was supported by a scholarship from the Monash Institute of Pharmaceutical Sciences, Monash University.
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Murphy, N.M., Pouton, C.W., Irving, H.R. (2017). High Molecular Weight DNA Enrichment with Peptide Nucleic Acid Probes. In: Tiemann-Boege, I., Betancourt, A. (eds) Haplotyping. Methods in Molecular Biology, vol 1551. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6750-6_4
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DOI: https://doi.org/10.1007/978-1-4939-6750-6_4
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