Abstract
Many short peptides selectively permeabilize the bacteria plasma membrane, leading to their lyses and death: they are therefore a source of antibacterial molecules and inspiration for novel and more selective drugs, which may have wider application in many other fields, as selective anticancer drugs. In this chapter, it is presented a new method to investigate the permeabilization properties of antimicrobial peptides under strict physiological conditions, employing the patch-clamp technique coupled to a fast perfusion system.
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Acknowledgment
Many thanks to Marco Aquila, Mascia Benedusi, Anna Fasoli, and Natascia Vedovato for helping in applying and refining this technique; Andrea Margutti manufactured many custom-made mechanical pieces. This work was supported by Ministero dell’Università e della Ricerca (MIUR), Roma, Italy, Project PRIN 2006 and 2008 (Progetti di Rilevante Interesse Nazionale), and by University of Ferrara, Project FAR 2006–2014 (Fondi di Ateneo per la Ricerca).
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Rispoli, G. (2017). Studying the Mechanism of Membrane Permeabilization Induced by Antimicrobial Peptides Using Patch-Clamp Techniques. In: Hansen, P. (eds) Antimicrobial Peptides. Methods in Molecular Biology, vol 1548. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6737-7_18
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DOI: https://doi.org/10.1007/978-1-4939-6737-7_18
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