Abstract
We describe a droplet microfluidics method to screen for multiple mutations of a same oncogene in a single experiment using passive droplet fusion. Genomic DNA from H1573 cell-line was screened for the presence of the six common mutations of the KRAS oncogene as well as wild-type sequences with a detection efficiency of 98 %. Furthermore, the mutant allelic fraction of the cell-line was also assessed correctly showing that the technique is quantitative
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Acknowledgments
This work was supported by Région Alsace, the Ministère de l’Enseignement Supérieur et de la Recherche, the Université de Strasbourg, the Université Paris Descartes, the Centre National de la Recherche Scientifique (CNRS), the Institut National de la Santé et de la Recherche Médicale (INSERM), the Institut National du Cancer (INCa, no. 2009–1-RT-03-US-1), and the Association pour la recherche sur le Cancer (ARC, no. SL220100601375).
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Pekin, D., Taly, V. (2017). Multiplex Detection of KRAS Mutations Using Passive Droplet Fusion. In: Taly, V., Viovy, JL., Descroix, S. (eds) Microchip Diagnostics. Methods in Molecular Biology, vol 1547. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6734-6_11
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DOI: https://doi.org/10.1007/978-1-4939-6734-6_11
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-6734-6
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