Abstract
The plant cell wall is composed of many complex polysaccharides. The composition and structure of the polysaccharides affect various cell properties including cell shape, cell function and cell adhesion. PACE (Polysaccharide Analysis using Carbohydrate gel Electrophoresis) uses a simple, rapid technique to analyze polysaccharide quantity and structure (Goubet et al., Anal Biochem 300:53–68, 2002).
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References
Goubet F, Jackson P, Deery MJ, Dupree P (2002) Polysaccharide analysis using carbohydrate gel electrophoresis: a method to study plant cell wall polysaccharides and polysaccharide hydrolases. Anal Biochem 300(1):53–68. doi:10.1006/abio.2001.5444
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Acknowledgments
With thanks to Dr. Ramana Pidatala for testing this version of the protocol and to my postdoctoral supervisor, Professor Paul Dupree, whose group developed PACE. This work was part of the DOE Joint BioEnergy Institute (http://www.jbei.org) supported by the U. S. Department of Energy, Office of Science, Office of Biological and Environmental Research, through contract DE-AC02-05CH11231 between Lawrence Berkeley National Laboratory and the U. S. Department of Energy..
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Mortimer, J.C. (2017). Structural Analysis of Cell Wall Polysaccharides Using PACE. In: de Lucas, M., Etchhells, J. (eds) Xylem. Methods in Molecular Biology, vol 1544. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6722-3_16
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DOI: https://doi.org/10.1007/978-1-4939-6722-3_16
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