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Xylem

Volume 1544 of the series Methods in Molecular Biology pp 223-231

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Structural Analysis of Cell Wall Polysaccharides Using PACE

  • Jennifer C. MortimerAffiliated withJoint BioEnergy Institute, Lawrence Berkeley National Laboratory Email author 

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Abstract

The plant cell wall is composed of many complex polysaccharides. The composition and structure of the polysaccharides affect various cell properties including cell shape, cell function and cell adhesion. PACE (Polysaccharide Analysis using Carbohydrate gel Electrophoresis) uses a simple, rapid technique to analyze polysaccharide quantity and structure (Goubet et al., Anal Biochem 300:53–68, 2002).

Key words

Electrophoresis Polysaccharides Glycosyl hydrolases Cell walls Fluorescent labelling 8-aminonaphthalene-1,3,6-trisulfonic acid Xylan