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Hypertension

Volume 1527 of the series Methods in Molecular Biology pp 311-320

Date:

Isolation and Differentiation of Human Macrophages

  • Francisco J. RiosAffiliated withInstitute of Cardiovascular and Medical Sciences, British Heart Foundation Glasgow Cardiovascular Research Centre, University of Glasgow Email author 
  • , Rhian M. TouyzAffiliated withKidney Research Centre, Department of Medicine, Ottawa Hospital Research Institute, University of OttawaInstitute of Cardiovascular and Medical Sciences, British Heart Foundation Glasgow Cardiovascular Research Centre, University of Glasgow
  • , Augusto C. MontezanoAffiliated withInstitute of Cardiovascular and Medical Sciences, British Heart Foundation Glasgow Cardiovascular Research Centre, University of Glasgow

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Abstract

Macrophage subtypes display protective or pathogenic activities in vascular lesions. They recognize and engulf modified lipids, accumulate in the arterial intima, contributing to the atherosclerosis development. In the heart, vascular tissues and perivascular adipose tissues, there is increasing evidence that macrophages play a role in endothelial dysfunction, vascular inflammation and remodeling. In this chapter we describe protocols to isolate human monocytes from peripheral blood mononuclear cell and how to differentiate them into macrophages by using growth factors (granulocyte-macrophage colony-stimulating factor—GM-CSF or colony-stimulating factor—M-CSF) or by different cell culture medium concentration. We also describe how to indentify different macrophage activation phenotypes.

Key words

Macrophage Monocyte Human Culture GM-CSF M-CSF