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Clickable Polymeric Coating for Glycan Microarrays

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Small Molecule Microarrays

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1518))

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Abstract

The interaction of carbohydrates with a variety of biological targets, including antibodies, proteins, viruses, and cells are of utmost importance in many aspects of biology. Glycan microarrays are increasingly used to determine the binding specificity of glycan-binding proteins. In this study, a novel microarray support is reported for the fabrication of glycan arrays that combines the higher sensitivity of a layered Si-SiO2 surface with a novel polymeric coating easily modifiable by subsequent click reaction. The alkyne-containing copolymer, adsorbed from an aqueous solution, produces a coating by a single step procedure and serves as a soft, tridimensional support for the oriented immobilization of carbohydrates via azide/alkyne Cu (I) catalyzed “click” reaction. The advantages of a functional 3D polymer coating making use of a click chemistry immobilization are combined with the high fluorescence sensitivity and superior signal-to-noise ratio of a Si-SiO2 substrate. The proposed approach enables the attachment of complex sugars on a silicon oxide surface by a method that does not require skilled personnel and chemistry laboratories.

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Authors and Affiliations

  1. Istituto di Chimica del Riconoscimento Molecolare, CNR, Via Mario Bianco 9, 20131, Milan, Italy

    Caterina Zilio, Laura Sola, Marina Cretich & Marcella Chiari

  2. Dipartimento di Chimica, Università degli Studi di Milano, Via Golgi 19, 20133, Milan, Italy

    Anna Bernardi

Authors
  1. Caterina Zilio
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  2. Laura Sola
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  3. Marina Cretich
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  4. Anna Bernardi
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  5. Marcella Chiari
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Corresponding author

Correspondence to Laura Sola .

Editor information

Editors and Affiliations

  1. DSO National Laboratories, Defence Medical and Environmental Resear, Singapore, Singapore

    Mahesh Uttamchandani

  2. Department of Chemistry Faculty of Science, National University of Singapore, Singapore, Singapore

    Shao Q. Yao

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Zilio, C., Sola, L., Cretich, M., Bernardi, A., Chiari, M. (2017). Clickable Polymeric Coating for Glycan Microarrays. In: Uttamchandani, M., Yao, S. (eds) Small Molecule Microarrays. Methods in Molecular Biology, vol 1518. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6584-7_5

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  • DOI: https://doi.org/10.1007/978-1-4939-6584-7_5

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6582-3

  • Online ISBN: 978-1-4939-6584-7

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