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An In Vitro Assay for Monitoring Topological DNA Entrapment by the Chromosomal Cohesin Complex

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Book cover Cohesin and Condensin

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1515))

Abstract

The cohesin complex is involved in a broad range of chromosomal biology, including DNA repair, gene transcription as well as sister chromatid cohesion. Cohesin is a large, ring-shaped protein complex and is thought to entrap DNA molecules inside of its ring. The unique DNA association is central to cohesin function and requires its ATPase and another heterodimer complex called the cohesin loader. Here we describe the biochemical reconstitution of topological cohesin loading onto DNA using the purified fission yeast cohesin proteins.

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Acknowledgement

This work was supported by the European Research Council and Japanese Society for the Promotion of Science (JSPS).

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Authors and Affiliations

  1. Institute of Innovative Research, Tokyo Institute of Technology, M6-11, 2-12-1, Ookayama, Megro, Tokyo, 152-8550, Japan

    Yasuto Murayama

  2. The Francis Crick Institute, Lincoln’s Inn Fields Laboratory, London, UK

    Frank Uhlmann

Authors
  1. Yasuto Murayama
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  2. Frank Uhlmann
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Corresponding author

Correspondence to Yasuto Murayama .

Editor information

Editors and Affiliations

  1. Department of Biological Chemistry, School of Medicine University of California— Irvine, Irvine, California, USA

    Kyoko Yokomori

  2. Laboratory of Genome Structure and Function Research Center for Epigenetic Disease, Institute of Molecular and Cellular Biosciences The University of Tokyo, Tokyo, Japan

    Katsuhiko Shirahige

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Murayama, Y., Uhlmann, F. (2017). An In Vitro Assay for Monitoring Topological DNA Entrapment by the Chromosomal Cohesin Complex. In: Yokomori, K., Shirahige, K. (eds) Cohesin and Condensin. Methods in Molecular Biology, vol 1515. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6545-8_2

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  • DOI: https://doi.org/10.1007/978-1-4939-6545-8_2

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6543-4

  • Online ISBN: 978-1-4939-6545-8

  • eBook Packages: Springer Protocols

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