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MiRNA Expression in Cystic Fibrosis Bronchial Epithelial Cells

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Book cover MicroRNA Profiling

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1509))

Abstract

Bronchial epithelial cells represent an invaluable tool to elucidate molecular signaling regulation in cystic fibrosis (CF). CF is a lethal genetic condition characterized by chronic inflammation in which bronchial epithelial cells play a pivotal role. Here we describe their use in analysis of microRNA (miRNA) and their target genes following a two-step RT-PCR miRNA profiling method in bronchial cell specimens from CF and control individuals where 667 human miRNA were examined. We also describe an approach to experimental modulation of these miRNA in vitro.

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Author information

Authors and Affiliations

  1. Royal College of Surgeons in Ireland, Education and Research Centre, Beaumont Hospital, Dublin, Ireland

    Irene K. Oglesby & Paul J. McKiernan

Authors
  1. Irene K. Oglesby
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  2. Paul J. McKiernan
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Corresponding author

Correspondence to Irene K. Oglesby .

Editor information

Editors and Affiliations

  1. Department of Science, Waterford Institute of Technology, Waterford, Ireland

    Sweta Rani

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© 2017 Springer Science+Business Media New York

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Oglesby, I.K., McKiernan, P.J. (2017). MiRNA Expression in Cystic Fibrosis Bronchial Epithelial Cells. In: Rani, S. (eds) MicroRNA Profiling. Methods in Molecular Biology, vol 1509. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6524-3_7

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  • DOI: https://doi.org/10.1007/978-1-4939-6524-3_7

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6522-9

  • Online ISBN: 978-1-4939-6524-3

  • eBook Packages: Springer Protocols

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