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MicroRNA Profiling of Exosomes

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MicroRNA Profiling

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1509))

Abstract

Exosomes are nano-sized membrane-bound vesicles released by a range of different cell types. Exosomes have been shown to specifically package certain membrane and cytosolic proteins and nucleic acids. Furthermore, it has been shown that their contents can be transferred to secondary cells, affecting the recipient cells’ cellular processes. Exosomes are present in a multitude of body fluids and so represent a novel source of circulating biomarkers. Here, we describe ultracentrifugation methods suitable for the isolation of exosomes from serum and plasma. We also detail transmission electron microscopy, nanoparticle tracking analysis, and immunoblotting methods suitable for the characterization of exosomes.

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Acknowledgements

Irish Cancer Society’s support of Breast-Predict [CCRC13GAL] and HEA PRTLI Cycle 5 funding of TBSI.

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Authors and Affiliations

  1. School of Pharmacy and Pharmaceutical Sciences & Trinity Biomedical Sciences Institute, Trinity College Dublin, Dublin 2, Ireland

    Melissa Daly & Lorraine O’Driscoll

Authors
  1. Melissa Daly
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  2. Lorraine O’Driscoll
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Corresponding author

Correspondence to Lorraine O’Driscoll .

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Editors and Affiliations

  1. Department of Science, Waterford Institute of Technology, Waterford, Ireland

    Sweta Rani

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© 2017 Springer Science+Business Media New York

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Daly, M., O’Driscoll, L. (2017). MicroRNA Profiling of Exosomes. In: Rani, S. (eds) MicroRNA Profiling. Methods in Molecular Biology, vol 1509. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6524-3_5

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  • DOI: https://doi.org/10.1007/978-1-4939-6524-3_5

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6522-9

  • Online ISBN: 978-1-4939-6524-3

  • eBook Packages: Springer Protocols

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