Abstract
Fluorescence live imaging is a powerful approach to study intracellular dynamics during cellular events such as cell division. By applying automated confocal live imaging to mouse oocytes, in which meiotic maturation can be induced in vitro after the introduction of fluorescent proteins through microinjection, the meiotic dynamics of intracellular structures, such as chromosomes, can be monitored at high resolution. A combination of this method with approaches for the perturbation of specific proteins opens up opportunities for understanding the molecular and intracellular basis of mammalian meiosis.
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Acknowledgements
We thank Dr. J. Ellenberg for a macro for automated confocal microscopy, the CDB imaging and animal facilities for supports. This work was supported grants from Nakajima Foundation, Uehara Memorial Foundation, and RIKEN CDB to T.S.K.
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Yoshida, S., Sakakibara, Y., Kitajima, T.S. (2016). Live Imaging of Intracellular Dynamics During Meiotic Maturation in Mouse Oocytes. In: Nezis, I. (eds) Oogenesis. Methods in Molecular Biology, vol 1457. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3795-0_18
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DOI: https://doi.org/10.1007/978-1-4939-3795-0_18
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-3795-0
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