Protocol

Natural Killer Cells

Volume 1441 of the series Methods in Molecular Biology pp 13-26

Date:

Application of Mass Cytometry (CyTOF) for Functional and Phenotypic Analysis of Natural Killer Cells

  • Alexander W. KayAffiliated withDepartment of Pediatrics, Stanford University School of Medicine
  • , Dara M. Strauss-AlbeeAffiliated withStanford Immunology, Stanford University School of Medicine
  • , Catherine A. BlishAffiliated withStanford Immunology, Stanford University School of MedicineDepartment of Medicine, Stanford University School of Medicine Email author 

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Abstract

Mass cytometry is a novel platform for high-dimensional phenotypic and functional analysis of single cells. This system uses elemental metal isotopes conjugated to monoclonal antibodies to evaluate up to 42 parameters simultaneously on individual cells with minimal overlap between channels. The platform can be customized for analysis of both phenotypic and functional markers. Here, we will describe methods to stain, collect, and analyze intracellular functional markers and surface phenotypic markers on natural killer cells.

Key words

CyTOF Mass cytometry Flow cytometry Natural killer cell Intracellular cytokine staining