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Endocannabinoid Signaling pp 95–101Cite as

Assay of GTPγS Binding in Autoradiography

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1412))

Abstract

Autoradiography of radiolabeled GTPγS ([35S]GTPγS) binding is a relevant method to study the function of G protein-coupled receptors (GPCRs), in tissue sections. Here, we describe the protocol for such a binding autoradiography, suitable to investigate the functionality of CB1 receptor in tissue slices from rodent brain.

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Author information

Author notes

    Authors and Affiliations

    1. Department of Biotechnology and Life Sciences, University of Insubria, Via Manara 7, 21052, Busto Arsizio (VA), Italy

      Marina Gabaglio, Pamela Prini, Erica Zamberletti, Tiziana Rubino & Daniela Parolaro

    2. Fondazione Zardi-Gori, via Cossa 1, Milano, Italy

      Daniela Parolaro

    Authors
    1. Marina Gabaglio
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    2. Pamela Prini
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    3. Erica Zamberletti
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    4. Tiziana Rubino
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    5. Daniela Parolaro
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    Corresponding author

    Correspondence to Daniela Parolaro .

    Editor information

    Editors and Affiliations

    1. Campus Bio-Medico University of Rome, Department of Medicine, Rome, Italy

      Mauro Maccarrone

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    © 2016 Springer Science+Business Media New York

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    Gabaglio, M., Prini, P., Zamberletti, E., Rubino, T., Parolaro, D. (2016). Assay of GTPγS Binding in Autoradiography. In: Maccarrone, M. (eds) Endocannabinoid Signaling. Methods in Molecular Biology, vol 1412. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3539-0_10

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    • DOI: https://doi.org/10.1007/978-1-4939-3539-0_10

    • Published:

    • Publisher Name: Humana Press, New York, NY

    • Print ISBN: 978-1-4939-3537-6

    • Online ISBN: 978-1-4939-3539-0

    • eBook Packages: Springer Protocols

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