Abstract
Current systems of recombinant protein production include bacterial, insect, and mammalian cell culture. However, these platforms are expensive to build and operate at commercial scales and/or have limited abilities to produce complex proteins. In recent years, plant-based expression systems have become top candidates for the production of recombinant proteins as they are highly scalable, robust, safe, and can produce complex proteins due to having a eukaryotic endomembrane system. Newly developed “deconstructed” viral vectors delivered via Agrobacterium tumefaciens (agroinfiltration) have enabled robust plant-based production of proteins with a wide range of applications. The leafy Lactuca sativa (lettuce) plant with its strong foundation in agriculture is an excellent host for pharmaceutical protein production. Here, we describe a method for agroinfiltration of lettuce that can rapidly produce high levels of recombinant proteins in a matter of days and has the potential to be scaled up to an agricultural level.
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Acknowledgement
The authors thank Dr. D. Green at Arizona State University for his continuous support for undergraduate research. This research was funded in part by the grant from the Bill & Melinda Gates Foundation OPP1043526 to Q. Chen. This research was also supported in part by funds from the School of Life Sciences Undergraduate Research (SOLUR) program at Arizona State University.
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Chen, Q. et al. (2016). Transient Protein Expression by Agroinfiltration in Lettuce. In: MacDonald, J., Kolotilin, I., Menassa, R. (eds) Recombinant Proteins from Plants. Methods in Molecular Biology, vol 1385. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3289-4_4
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DOI: https://doi.org/10.1007/978-1-4939-3289-4_4
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