Protocol

Suppression and Regulation of Immune Responses

Volume 1371 of the series Methods in Molecular Biology pp 225-238

Standardized Multi-Color Flow Cytometry and Computational Biomarker Discovery

  • Stephan SchlickeiserAffiliated withInstitute of Medical Immunology, Charité University Medicine 
  • , Mathias StreitzAffiliated withInstitute of Medical Immunology, Charité University Medicine 
  • , Birgit SawitzkiAffiliated withInstitute of Medical Immunology, Charité University Medicine Email author 

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Abstract

Multi-color flow cytometry has become a valuable and highly informative tool for diagnosis and therapeutic monitoring of patients with immune deficiencies or inflammatory disorders. However, the method complexity and error-prone conventional manual data analysis often result in a high variability between different analysts and research laboratories. Here, we provide strategies and guidelines aiming at a more standardized multi-color flow cytometric staining and unsupervised data analysis for whole blood patient samples.

Key words

Flow cytometry Immune monitoring Standardization Data analysis