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Peptide-Carrier Conjugation

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Peptide Antibodies

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1348))

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Abstract

To produce antibodies against synthetic peptides it is necessary to couple them to a protein carrier. This chapter provides a nonspecialist overview of peptide-carrier conjugation. Furthermore, a protocol for coupling cysteine-containing peptides to bovine serum albumin is outlined.

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References

  1. Lee B-S, Huang J-S, Jayathilaka GDL et al (2010) Production of antipeptide antibodies. Methods Mol Biol 657:93–108

    Article  CAS  PubMed  Google Scholar 

  2. Muller S (1999) Peptide Carrier Conjugation In: van Regenmortel MVH and Muller S (eds) Synthetic Peptides as Antigens, Elsevier, Amsterdam pp. 79–130

    Google Scholar 

  3. Hermanson GT (2013) Bioconjugate techniques, 3rd edn. Academic, New York

    Google Scholar 

  4. Strynadka NC, Redmond MJ, Parker R et al (1988) Use of synthetic peptides to map the antigenic determinants of glycoprotein D of herpes simplex virus. J Virol 62:3474–3483

    PubMed Central  CAS  PubMed  Google Scholar 

  5. Briand JP, Muller S, van Regenmortel MHV (1985) Synthetic peptides as antigens: pitfalls of conjugation methods. J Immunol Methods 78:59–69

    Article  CAS  PubMed  Google Scholar 

  6. Carlsson J, Drevin H, Axén R (1978) Protein thiolation and reversible protein-protein conjugation. SPDP, a New heterobifunctional reagent. Biochem J 173:723–737

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  7. Lateef SS, Gupta S, Jayathilaka LP et al (2007) An improved protocol for coupling synthetic peptides to carrier proteins for antibody production using DMF to solubilize peptides. J Biomol Tech 18:173–176

    PubMed Central  PubMed  Google Scholar 

  8. Plaué S, Muller S, Briand JP et al (1990) Recent advances in solid-phase peptide synthesis and preparation of antibodies to synthetic peptides. Biologicals 18:147–157

    Article  PubMed  Google Scholar 

  9. Goodfriend, T. L., Levine, L. Fasman, G. D. (1964). Antibodies to bradykinin and angiotensin: a use of carbodiimides in immunology. Science 144:1344–1346

    Google Scholar 

  10. Rector ES, Schwenk RJ, Tse KS et al (1978) A method for the preparation of protein-protein conjugates of predetermined composition. J Immunol Methods 24:321–336

    Article  CAS  PubMed  Google Scholar 

  11. Kitagawa T, Aikawa T (1976) Enzyme coupled immunoassay of insulin using a novel coupling reagent. J Biochem 76:233–236

    Google Scholar 

  12. Houen G, Olsen DT, Hansen PR et al (2003) Preparation of bioconjugates by solid-phase conjugation to ion exchange matrix-adsorbed carrier proteins. Bioconjug Chem 1376:75–79

    Article  Google Scholar 

  13. Schaaper WMM, Lankof H, Puijk WC et al (1989) Manipulation of antipeptide immune response by varying the coupling of the peptide with the carrier protein. Mol Immunol 26:81–85

    Article  CAS  PubMed  Google Scholar 

  14. Tam JP (1988) Synthetic peptide vaccine design: synthesis and properties of a high-density multiple antigenic peptide system. Proc Natl Acad Sci U S A 85:5409–5413

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  15. Kowalczyk W, Monsó M, de la Torre BG et al (2011) Synthesis of multiple antigenic peptides (MAPs)—strategies and limitations. J Peptide Sci 17:247–251

    Article  CAS  Google Scholar 

  16. Tam JP, Lu Y-A (1989) Vaccine engineering: enhancement of immunogenicity of synthetic peptide vaccines related to Hepatitis in chemical defined models consisting of T- and B-cell epitopes. Proc Natl Acad Sci U S A 86:9084–9088

    Article  PubMed Central  CAS  PubMed  Google Scholar 

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Correspondence to Paul R. Hansen .

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Hansen, P.R. (2015). Peptide-Carrier Conjugation. In: Houen, G. (eds) Peptide Antibodies. Methods in Molecular Biology, vol 1348. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2999-3_6

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  • DOI: https://doi.org/10.1007/978-1-4939-2999-3_6

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2998-6

  • Online ISBN: 978-1-4939-2999-3

  • eBook Packages: Springer Protocols

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