Abstract
Understanding synaptic connectivity patterns between neurons and fine anatomical features of neural circuits represent key steps in our journey to unravel brain connectomes . In the last few years the development of new imaging tools and technologies has significantly improved our understanding of neural circuits. A remarkable example of this is a high-resolution immunofluorescence technique called array tomography (AT ). AT combines conventional immunofluorescence techniques to label antigens with ultrathin (70 nm) serial sectioning, and 3D reconstruction . Thus, serial images can be aligned and rendered into volumetric images suitable for semi-automated quantitative analysis using the ImageJ software. AT allows antibody elution and multiple rounds of immunolabeling , offering the unique possibility of examining the spatial distribution of multiple antigens and their relationships to each other in the same tissue volume . AT represents a new powerful and quantitative tool, particularly useful to characterize molecular components of synapses as well as to explore fine anatomical features within the synaptic neuropil .
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Soiza-Reilly, M. (2015). Array Tomography: A Novel High-Resolution Immunofluorescence Technique. In: Merighi, A., Lossi, L. (eds) Immunocytochemistry and Related Techniques. Neuromethods, vol 101. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2313-7_20
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DOI: https://doi.org/10.1007/978-1-4939-2313-7_20
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