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Mobile Health Technologies pp 57–69Cite as

Immunofluorescence Microtip Sensor for Point-of-Care Tuberculosis (TB) Diagnosis

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1256))

Abstract

A immunofluorescence microtip sensor was developed for specific detection of Mycobacterium cells in sputum samples by the combination of electric field, streaming flow, and immuno-affinity binding. The detection limit was 200 CFU/mL in human sputum, which was comparable to PCR but without requiring bacteriological culture, centrifugation, or nucleic acid amplification. In spite of the complex nature of physical, chemical, and biological mechanisms, the simple operation of “dipping and withdrawal” of tips will allow for screening by minimally trained personnel within 30 min. In addition, the minimal power requirement (5 W) combined with low assay cost is ideal for point-of-care (POC) screening in resource-limited settings.

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Acknowledgements

We acknowledge the support of CDC SBIR II Contract (200-2009-31946) and the grant from the Catalysis Foundation for Health. In addition, J.C., J.K., and W.Y. acknowledge the support of NSF Career (ECCS-0846454). We appreciate valuable discussion with Hyun-Boo Lee about the concentration mechanism.

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Authors and Affiliations

  1. Department of Mechanical Engineering, University of Washington, Seattle, WA, USA

    Jong-Hoon Kim & Jae-Hyun Chung

  2. NanoFacture Inc., 52651, Bellevue, WA, USA

    Kyong-Hoon Lee

  3. Department of Environmental and Occupational Health Sciences, University of Washington, Seattle, WA, USA

    Gerard A. Cangelosi

Authors
  1. Jong-Hoon Kim
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  2. Kyong-Hoon Lee
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  3. Gerard A. Cangelosi
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  4. Jae-Hyun Chung
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Corresponding author

Correspondence to Jae-Hyun Chung .

Editor information

Editors and Affiliations

  1. National Cancer Institute, Rockville, Maryland, USA

    Avraham Rasooly

  2. Fischell Department of Bioengineering, University of Maryland, College Park, Maryland, USA

    Keith E. Herold

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Kim, JH., Lee, KH., Cangelosi, G.A., Chung, JH. (2015). Immunofluorescence Microtip Sensor for Point-of-Care Tuberculosis (TB) Diagnosis. In: Rasooly, A., Herold, K. (eds) Mobile Health Technologies. Methods in Molecular Biology, vol 1256. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2172-0_4

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  • DOI: https://doi.org/10.1007/978-1-4939-2172-0_4

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2171-3

  • Online ISBN: 978-1-4939-2172-0

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