Abstract
Phage display enables the synthesis, selection, and screening of large, polypeptide libraries (>1 × 1010 different members). Selections from such libraries can identify binding partners to essentially any desired target (Sarikaya et al., Annu Rev Mater Res 34:373–408, 2004; Deutscher, Chem Rev 110:3196–3211, 2010). Peptides with affinity or reactivity to small molecule probes are attractive for numerous uses including the targeted, site-specific labeling of proteins. Here, we describe selection and screening protocols for the identification of short peptides that can selectively bind to and/or react with small molecules.
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Acknowledgment
We gratefully acknowledge the support of the NIGMS of the NIH (1 R01 GM100700-01 and 1 R01 GM078528-01).
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Eldridge, G.M., Weiss, G.A. (2015). Identifying Reactive Peptides from Phage-Displayed Libraries. In: Derda, R. (eds) Peptide Libraries. Methods in Molecular Biology, vol 1248. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2020-4_13
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DOI: https://doi.org/10.1007/978-1-4939-2020-4_13
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2019-8
Online ISBN: 978-1-4939-2020-4
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