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Measuring Intranuclear and Nuclear Envelope [Ca2+] vs. Cytosolic [Ca2+]

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Nuclear G-Protein Coupled Receptors

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1234))

Abstract

Nuclear Ca2+ regulates key cellular processes, including gene expression, apoptosis, assembly of the nuclear envelope, and nucleocytoplasmic transport. Quantification of subcellularly resolved Ca2+ signals is, therefore, essential for understanding physiological and pathological processes in various cell types. However, the properties of commonly used Ca2+-fluorescent indicators in intracellular compartments may differ, thus affecting the translation of Ca2+-dependent fluorescence changes into quantitative changes of Ca2+ concentration. Here, we describe technical approaches for reliable subcellular quantification of [Ca2+] in the cytoplasm vs. the nucleus and the nuclear envelope by in situ calibration of fluorescein-derived fluorescent indicators Fluo-4 and Fluo-5N.

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Author information

Authors and Affiliations

  1. Department of Pharmacology, University of California Davis, Davis, CA, 95616-8636, USA

    Senka Ljubojević

  2. Department of Cardiology, Medical University of Graz, Graz, Austria

    Senka Ljubojević

  3. Department of Pharmacology, University of California, Genome Building Rm 3513, 451 E. Health Sciences Dr., Davis, CA, 95616-8636, USA

    Donald M. Bers

Authors
  1. Senka Ljubojević
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  2. Donald M. Bers
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Corresponding author

Correspondence to Donald M. Bers .

Editor information

Editors and Affiliations

  1. Montreal Heart Institute & Université de Montréal, Montreal, Québec, Canada

    Bruce G. Allen

  2. Pharmacology and Therapeutics, McGill University, Montreal, Québec, Canada

    Terence E. Hébert

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Ljubojević, S., Bers, D.M. (2015). Measuring Intranuclear and Nuclear Envelope [Ca2+] vs. Cytosolic [Ca2+]. In: Allen, B., Hébert, T. (eds) Nuclear G-Protein Coupled Receptors. Methods in Molecular Biology, vol 1234. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1755-6_12

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  • DOI: https://doi.org/10.1007/978-1-4939-1755-6_12

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1754-9

  • Online ISBN: 978-1-4939-1755-6

  • eBook Packages: Springer Protocols

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