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Histochemical Analysis of Heparan Sulfate 3-O-Sulfotransferase Expression in Mouse Brain

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Book cover Glycosaminoglycans

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1229))

Abstract

In situ hybridization provides information for understanding the localization of gene expression in various tissues. The relative expression levels of mRNAs in a single cell can be sensitively visualized by this technique. Furthermore, since in situ hybridization is a histological technique, tissue structure is maintained after fixation, and it is possible to accurately identify cell types. We have examined the expression of heparan sulfate sulfotransferases by in situ hybridization to better understand the functions of heparan sulfate in the development of mouse nervous system. This chapter describes methods of in situ hybridization analyses using cRNA probes labeled with nonradioactive nucleotides.

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References

  1. Bernfield M, Gotte M, Park PW, Reizes O, Fitzgerald ML, Lincecum J, Zako M (1999) Functions of cell surface heparan sulfate proteoglycans. Annu Rev Biochem 68:729–777

    Article  CAS  PubMed  Google Scholar 

  2. Kreuger J, Kjellén L (2012) Heparan sulfate biosynthesis: regulation and variability. J Histochem Cytochem 60:898–907

    Article  PubMed Central  PubMed  Google Scholar 

  3. Lindahl U, Kjellén L (2013) Pathophysiology of heparan sulphate: many diseases, few drugs. J Intern Med 273:555–571

    Article  CAS  PubMed  Google Scholar 

  4. Mizumoto S, Sugahara K (2013) Glycosaminoglycans are functional ligands for receptor for advanced glycation end-products in tumors. FEBS J 280:2462–2470

    Article  CAS  PubMed  Google Scholar 

  5. Tiwari V, Maus E, Sigar IM, Ramsey KH, Shukla D (2012) Role of heparan sulfate in sexually transmitted infections. Glycobiology 22:1402–1412

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  6. Kinnunen T, Huang Z, Townsend J, Gatdula MM, Brown JR, Esko JD, Turnbull JE (2005) Heparan 2-O-sulfotransferase, hst-2, is essential for normal cell migration in Caenorhabditis elegans. Proc Natl Acad Sci U S A 102:1507–1512

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  7. Esko JD, Selleck SB (2002) Order out of chaos: assembly of ligand binding sites in heparan sulfate. Annu Rev Biochem 71:435–471

    Article  CAS  PubMed  Google Scholar 

  8. Yabe T, Hata T, He J, Maeda N (2005) Developmental and regional expression of heparan sulfate sulfotransferase genes in the mouse brain. Glycobiology 15:982–993

    Article  CAS  PubMed  Google Scholar 

  9. Cox KH, DeLeon DV, Angerer LM, Angerer RC (1984) Detection of mRNAs in sea urchin embryos by in situ hybridization using asymmetric RNA probes. Dev Biol 101:485–502

    Article  CAS  PubMed  Google Scholar 

  10. Wilcox JN, Gee CE, Roberts JL (1986) In situ cDNA:mRNA hybridization: development of a technique to measure mRNA levels in individual cells. Methods Enzymol 124:510–533

    Article  CAS  PubMed  Google Scholar 

  11. Shworak NW, Liu J, Fritze LM, Schwartz JJ, Zhang L, Logeart D, Rosenberg RD (1997) Molecular cloning and expression of mouse and human cDNAs encoding heparan sulfate d-glucosaminyl 3-O-sulfotransferase. J Biol Chem 272:28008–28019

    Article  CAS  PubMed  Google Scholar 

  12. Shworak NW, Liu J, Petros LM, Zhang L, Kobayashi M, Copeland NG, Jenkins NA, Rosenberg RD (1999) Multiple isoforms of heparan sulfate d-glucosaminyl 3-O-sulfotransferase. Isolation, characterization, and expression of human cDNAs and identification of distinct genomic loci. J Biol Chem 274:5170–5184

    Article  CAS  PubMed  Google Scholar 

  13. Shukla D, Liu J, Blaiklock P, Shworak NW, Bai X, Esko JD, Cohen GH, Eisenberg RJ, Rosenberg RD, Spear PG (1999) A novel role for 3-O-sulfated heparan sulfate in herpes simplex virus 1 entry. Cell 99:13–22

    Article  CAS  PubMed  Google Scholar 

  14. Lawrence R, Yabe T, Hajmohammadi S, Rhodes J, McNeely M, Liu J, Lamperti ED, Toselli PA, Lech M, Spear PG, Rosenberg RD, Shworak NW (2007) The principal neuronal gD-type 3-O-sulfotransferases and their products in central and peripheral nervous system tissues. Matrix Biol 26:442–455

    Article  CAS  PubMed Central  PubMed  Google Scholar 

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Author information

Authors and Affiliations

  1. Faculty of Applied Biological Sciences, Department of Applied Life Science, Gifu University, 1-1 Yanagido, Gifu, 501-1193, Japan

    Tomio Yabe

  2. Tokyo Metropolitan Institute of Medical Science, 2-1-6 Kamikitazawa, Setagaya-ku, Tokyo, 156-8506, Japan

    Nobuaki Maeda

Authors
  1. Tomio Yabe
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  2. Nobuaki Maeda
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Corresponding author

Correspondence to Tomio Yabe .

Editor information

Editors and Affiliations

  1. Department of Medicinal Chemistry, University of Utah, Salt Lake City, Utah, USA

    Kuberan Balagurunathan

  2. Department of Genetics, Cell Biology and Development, The University of Minnesota, Minneapolis, Minnesota, USA

    Hiroshi Nakato

  3. Department of Medicinal Chemistry, Virginia Commonwealth University, Richmond, Virginia, USA

    Umesh R. Desai

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Yabe, T., Maeda, N. (2015). Histochemical Analysis of Heparan Sulfate 3-O-Sulfotransferase Expression in Mouse Brain. In: Balagurunathan, K., Nakato, H., Desai, U. (eds) Glycosaminoglycans. Methods in Molecular Biology, vol 1229. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1714-3_29

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  • DOI: https://doi.org/10.1007/978-1-4939-1714-3_29

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1713-6

  • Online ISBN: 978-1-4939-1714-3

  • eBook Packages: Springer Protocols

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