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T-Helper Cells

Volume 1193 of the series Methods in Molecular Biology pp 55-68

Date:

Mass Cytometry Analysis of Human T Cell Phenotype and Function

  • Evan W. NewellAffiliated withSingapore Immunology Network (SIgN), Agency for Science Technology and Research (A-STAR) Email author 
  • , Lai Li YunAffiliated withSingapore Immunology Network (SIgN), Agency for Science Technology and Research (A-STAR)

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Abstract

Mass cytometry is a form of flow cytometry based on single-cell mass spectrometry that uses monoisotopic elemental labels to probe individual cells. Reduced cross talk between channels and an ability to measure >30 independent cellular parameters make this an attractive approach for high-dimensional analysis of cellular phenotypes and function. Here, methods of using this approach for the analysis of human T cell surface markers and intracellular cytokines are described.

Key words

Mass cytometry Cytometry by time-of-flight (CyTOF) Flow cytometry Intracellular cytokine staining