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The Characterization of Living Bacterial Colonies Using Nanospray Desorption Electrospray Ionization Mass Spectrometry

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Engineering and Analyzing Multicellular Systems

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1151))

Abstract

Nanospray desorption electrospray ionization (nano-DESI) coupled with high-resolution mass spectrometry (MS) and tandem mass spectrometry (MS/MS) enable detailed molecular characterization of living bacterial colonies directly from nutrient agar. The ability to detect molecular signatures of living microbial communities is important for investigating metabolic exchange between species without affecting the viability of the colonies. We describe the protocol for bacterial growth, sample preparation, ambient profiling, and data analysis of microbial communities using nano-DESI MS.

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Acknowledgements

The research described in this paper is part of the Chemical Imaging Initiative (CII) at Pacific Northwest National Laboratory (PNNL). It was conducted under the Laboratory Directed Research and Development (LDRD) Program at PNNL, a multiprogram national laboratory operated by Battelle for the U.S. Department of Energy (DOE). J.L., B.S.H., M.J.M. acknowledge support from the CII at PNNL. B.S.H. was supported in part by the DOE Science Undergraduate Laboratory Internship (SULI) at PNNL. The authors acknowledge fruitful discussions with Drs. Jim Fredrickson and Margie Romine (PNNL). A portion of this research was performed using EMSL, a national scientific user facility sponsored by DOE Office of Biological and Environmental Research (OBER) and located at PNNL. PNNL is operated by Battelle for DOE.

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Authors and Affiliations

  1. Physical Sciences Division, Pacific Northwest National Laboratory, 999, MSIN K8-88, Richland, WA, 99352, USA

    Brandi S. Heath & Julia Laskin

  2. Biological Sciences Division, Pacific Northwest National Laboratory, 999, MSIN K8-88, Richland, WA, 99352, USA

    Matthew J. Marshall

Authors
  1. Brandi S. Heath
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  2. Matthew J. Marshall
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  3. Julia Laskin
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Corresponding author

Correspondence to Julia Laskin .

Editor information

Editors and Affiliations

  1. School of Life Sciences, University of Science & Technology of China,, Hefei, Anhui, China

    Lianhong Sun

  2. Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA

    Wenying Shou

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Heath, B.S., Marshall, M.J., Laskin, J. (2014). The Characterization of Living Bacterial Colonies Using Nanospray Desorption Electrospray Ionization Mass Spectrometry. In: Sun, L., Shou, W. (eds) Engineering and Analyzing Multicellular Systems. Methods in Molecular Biology, vol 1151. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0554-6_14

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  • DOI: https://doi.org/10.1007/978-1-4939-0554-6_14

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-0553-9

  • Online ISBN: 978-1-4939-0554-6

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