Abstract
The antinuclear antibodies (ANA) also known as antinuclear factors (ANF) are unwanted molecules which bind and destroy certain structures within the nucleus. In systemic lupus erythematosus (SLE), they are produced in excess; hence their detection in the blood of patients is important for diagnosis and monitoring of the disease. Several methods are available which can be used to detect ANA; nevertheless, indirect immunofluorescence antinuclear antibody test (IF-ANA) is considered a “reference method” for their detection. Though IF-ANA is relatively easier to perform, its interpretation requires considerable skill and experience. The chapter therefore is aimed to provide comprehensive details to readers, not only about its methodology but also the result interpretation and reporting aspects of IF-ANA.
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References
Mutasim DF, Adams BB (2000) A practical guide for serologic evaluation of autoimmune connective tissue diseases. J Am Acad Dermatol 42:159–174, quiz 174–156
Rekvig OP, Kalaaji M, Nossent H (2004) Anti-DNA antibody subpopulations and lupus nephritis. Autoimmun Rev 3:1–6
Tan EM, Cohen AS, Fries JF, Masi AT, Mcshane DJ, Rothfield NF et al (1982) The 1982 revised criteria for the classification of systemic lupus erythematosus. Arthritis Rheum 25:1271–1277
Tan EM (1982) Autoantibodies to nuclear antigens (ANA): their immunobiology and medicine. Adv Immunol 33:167–240
Tan EM (1989) Antinuclear antibodies: diagnostic markers for autoimmune diseases and probes for cell biology. Adv Immunol 44:93–151
Kumar Y, Bhatia A, Minz RW (2009) Antinuclear antibodies and their detection methods in diagnosis of connective tissue diseases: a journey revisited. Diagn Pathol 4:1
Egner W (2000) The use of laboratory tests in the diagnosis of SLE. J Clin Pathol 53:424–432
Kavanaugh A, Tomar R, Reveille J, Solomon DH, Homburger HA (2000) Guidelines for clinical use of the antinuclear antibody test and tests for specific autoantibodies to nuclear antigens. American College of Pathologists. Arch Pathol Lab Med 124:71–81
Gonzalez EN, Rothfield NF (1966) Immunoglobulin class and pattern of nuclear fluorescence in systemic lupus erythematosus. N Engl J Med 274:1333–1338
Tonutti E, Visentini D, Bizzaro N (2007) Interpretative comments on autoantibody tests. Autoimmun Rev 6:341–346
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Kumar, Y., Bhatia, A. (2014). Detection of Antinuclear Antibodies in SLE. In: Eggleton, P., Ward, F. (eds) Systemic Lupus Erythematosus. Methods in Molecular Biology, vol 1134. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0326-9_3
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DOI: https://doi.org/10.1007/978-1-4939-0326-9_3
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Publisher Name: Humana Press, New York, NY
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