Abstract
Blue native electrophoresis (BN-PAGE) is a highly resolutive method suited to the study of high molecular weight protein complexes between 100 and >3000 kDa. One of the drawbacks of this method is that it is very time-consuming and requires high quantities of purified organelles. Here we describe a high throughput BN-PAGE method allowing to screen libraries of plants potentially altered in respiratory metabolism.
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Vincis Pereira Sanglard, L., Colas des Francs-Small, C. (2022). High-Throughput BN-PAGE for Mitochondrial Respiratory Complexes . In: Van Aken, O., Rasmusson, A.G. (eds) Plant Mitochondria. Methods in Molecular Biology, vol 2363. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1653-6_10
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DOI: https://doi.org/10.1007/978-1-0716-1653-6_10
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