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Introduction to Genetic, Genomic and System Analyses of Pure Cultures

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Hydrocarbon and Lipid Microbiology Protocols

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Abstract

Although considered to be only a minute fraction of global biodiversity, the handling of the culturable microbiota is still essential for exploring the interface of the bacterial world with lipids, hydrocarbons and other chemicals. This endeavour requires a suite of wet and computational tools that are the subject of the present volume. The protocols detailed below go from generating large volumes of data with a suite of omics to methods for distilling such a data into information and this in turn into new and useful knowledge. To this end, in silico approaches have to go hand-in-hand with new strategies for genome editing and controlled perturbations of the systems under study.

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Acknowledgments

The work in Author’s Laboratory is supported by the CAMBIOS Project of the Spanish Ministry of Economy and Competitiveness, the ARISYS, EVOPROG and EMPOWERPUTIDA Contracts of the EU, The ERANET-IB and the PROMT Project of the CAM.

Author information

Authors and Affiliations

  1. Systems Biology Program, Centro Nacional de Biotecnología CSIC, Campus de Cantoblanco, Darwin 3, 28049, Madrid, Spain

    Víctor de Lorenzo

Authors
  1. Víctor de Lorenzo
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Correspondence to Víctor de Lorenzo .

Editor information

Editors and Affiliations

  1. School of Biological Sciences, University of Essex Dept. Biological Sciences, Colchester, Essex, United Kingdom

    Terry J. McGenity

  2. Institute of Microbiology, Technical University Braunschweig, Braunschweig, Germany

    Kenneth N. Timmis

  3. Department of Biology, University of the Balearic Islands and Mediterranean Institute for Advanced Studies (IMEDEA, UIB-CSIC), Palma de Mallorca, Spain

    Balbina Nogales

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© 2016 Springer-Verlag Berlin Heidelberg

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de Lorenzo, V. (2016). Introduction to Genetic, Genomic and System Analyses of Pure Cultures. In: McGenity, T., Timmis, K., Nogales , B. (eds) Hydrocarbon and Lipid Microbiology Protocols. Springer Protocols Handbooks. Springer, Berlin, Heidelberg. https://doi.org/10.1007/8623_2016_192

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  • DOI: https://doi.org/10.1007/8623_2016_192

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  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-662-50433-8

  • Online ISBN: 978-3-662-50435-2

  • eBook Packages: Springer Protocols

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