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Mitophagy pp 9–17Cite as

Induction of PINK1/Parkin-Mediated Mitophagy

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1759))

Abstract

PINK1/Parkin mitophagy is a key mechanism to contribute mitochondrial quality control, and the defects are thought to be a cause of those Parkinson’s disease onsets. Upon loss of mitochondrial membrane potential, PINK1 and Parkin are activated to promote the proteasomal degradation of mitochondrial outer membrane proteins and selective elimination of damaged mitochondria by autophagy. In this chapter, we describe the methods for induction of PINK1/Parkin-mediated mitophagy in tissue culture cell lines.

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Author information

Authors and Affiliations

  1. Department of Neurology, Juntendo University Graduate School of Medicine, Bunkyo-ku, Tokyo, 113-8421, Japan

    Shigeto Sato

  2. Department of Neurology, Juntendo University School of Medicine, Hongo, Bunkyo, Tokyo, Japan

    Norihiko Furuya

Authors
  1. Shigeto Sato
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  2. Norihiko Furuya
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Corresponding author

Correspondence to Norihiko Furuya .

Editor information

Editors and Affiliations

  1. Department of Neurology, Juntendo University School of Medicine, Tokyo, Japan

    Nobutaka Hattori

  2. Department of Neurology, Juntendo University School of Medicine, Tokyo, Japan

    Shinji Saiki

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Sato, S., Furuya, N. (2017). Induction of PINK1/Parkin-Mediated Mitophagy. In: Hattori, N., Saiki, S. (eds) Mitophagy. Methods in Molecular Biology, vol 1759. Humana Press, New York, NY. https://doi.org/10.1007/7651_2017_7

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  • DOI: https://doi.org/10.1007/7651_2017_7

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7749-9

  • Online ISBN: 978-1-4939-7750-5

  • eBook Packages: Springer Protocols

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