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Part of the series Methods in Molecular Biology pp 1-12

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Monitoring of Iron Depletion-Induced Mitophagy in Pathogenic Yeast

  • Koichi TanabeAffiliated withDepartment of Food Science and Human Nutrition, Faculty of Agriculture, Ryukoku UniversityDepartment of Chemotherapy and Mycoses, National Institute of Infectious Diseases Email author 
  • , Minoru NagiAffiliated withDepartment of Chemotherapy and Mycoses, National Institute of Infectious Diseases

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Abstract

Mitophagy, which is the degradation of mitochondria via selective autophagic machinery, is thought to be involved in regulating the mass and function of mitochondria. Methods for detection of mitophagy have been reported for several fungal cells including some budding yeast, methylotrophic yeast, and filamentous fungi. Mitophagy in Saccharomyces cerevisiae is activated under nitrogen-poor conditions; however, the regulatory mechanism of mitophagy in most fungi has not been elucidated. Here we describe methods to monitor mitophagy in the pathogenic yeast Candida glabrata under iron-depleted conditions but not under nitrogen starvation. This observation may provide some clues to elucidate the physiological roles of mitophagy in eukaryotes.

Keywords

Atg32 Autophagy Candida Iron Mitochondria Mitophagy Pathogenicity Yeast