Abstract
Mitophagy is a cellular process of autophagy-based mitochondrial degradation that eliminates dysfunctional mitochondria and ensures cellular homeostasis. In pancreatic islet β-cells, mitochondria play a pivotal role in glucose-stimulated insulin secretion through ATP production from glucose oxidation. Recent studies have shown that impaired mitophagy and the subsequent mitochondrial compromise contribute to β-cell dysfunction and glucose intolerance. In this chapter, we describe a protocol to monitor Parkin-mediated mitophagy in pancreatic MIN6 β-cells using flow cytometry and a pH-sensitive fluorophore, mKeima.
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Acknowledgements
This study was supported in part by Grants-in-Aid from the Ministry of Education, Science and Culture of Japan (MEXT) (KAKENHI 15K09144 to SM).
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Hoshino, A., Matoba, S. (2017). Observation of Parkin-Mediated Mitophagy in Pancreatic β-Cells. In: Hattori, N., Saiki, S. (eds) Mitophagy. Methods in Molecular Biology, vol 1759. Humana Press, New York, NY. https://doi.org/10.1007/7651_2017_39
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DOI: https://doi.org/10.1007/7651_2017_39
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Publisher Name: Humana Press, New York, NY
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