Protocol

Part of the series Methods in Molecular Biology pp 1-6

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Short Overview

  • Norihiko FuruyaAffiliated withDepartment of Neuroscience for Neurodegenerative Disorders, Juntendo University Graduate School of MedicineDepartment of Neurology, Juntendo University Graduate School of Medicine Email author 

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Abstract

Mitochondrial autophagy (mitophagy) is a mitochondrial quality control mechanism that selectively removes damaged mitochondria via autophagic degradation. Autophagic adaptor/receptor proteins contribute to the selective degradation of damaged mitochondria by autophagy. A part of them containing both ubiquitin binding domains and Atg8 interacting motif (AIM)/LC3 interacting region (LIR) motifs, which bind to the autophagy-related protein 8 (Atg8) family (LC3 and GABARAP family), lead ubiquitylated (damaged) mitochondria to selective removal. On the other hand, some specific outer mitochondrial membrane-anchored proteins containing AIM/LIR motif function as another type of autophagy adaptor/receptor proteins. Here I briefly summarize mechanisms of mitophagy and its related proteins.

Keywords

Atg8 interacting motif (AIM)/LC3 interacting region (LIR) Autophagy adaptor Mitophagy