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3D-Printing Composite Polycaprolactone-Decellularized Bone Matrix Scaffolds for Bone Tissue Engineering Applications

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Decellularized Scaffolds and Organogenesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1577))

Abstract

Millions of patients worldwide require bone grafts for treatment of large, critically sized bone defects from conditions such as trauma, cancer, and congenital defects. Tissue engineered (TE) bone grafts have the potential to provide a more effective treatment than current bone grafts since they would restore fully functional bone tissue in large defects. Most bone TE approaches involve a combination of stem cells with porous, biodegradable scaffolds that provide mechanical support and degrade gradually as bone tissue is regenerated by stem cells. 3D-printing is a key technique in bone TE that can be used to fabricate functionalized scaffolds with patient-specific geometry. Using 3D-printing, composite polycaprolactone (PCL) and decellularized bone matrix (DCB) scaffolds can be produced to have the desired mechanical properties, geometry, and osteoinductivity needed for a TE bone graft. This book chapter will describe the protocols for fabricating and characterizing 3D-printed PCL:DCB scaffolds. Moreover, procedures for culturing adipose-derived stem cells (ASCs) in these scaffolds in vitro will be described to demonstrate the osteoinductivity of the scaffolds.

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Author notes

  1. Alexandra N. Rindone and Ethan Nyberg contributed equally to this work.

Authors and Affiliations

  1. Translational TE Center, Johns Hopkins University School of Medicine, Baltimore, MD, 21287, USA

    Alexandra N. Rindone, Ethan Nyberg & Warren L. Grayson

  2. Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, MD, 21205, USA

    Alexandra N. Rindone, Ethan Nyberg & Warren L. Grayson

  3. Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, MD, 21218, USA

    Warren L. Grayson

  4. Institute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD, 21218, USA

    Warren L. Grayson

  5. Johns Hopkins University, Translational Tissue Engineering Center, 400 N. Broadway, Smith 5023, Baltimore, MD, 21231, USA

    Warren L. Grayson

Authors
  1. Alexandra N. Rindone
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  2. Ethan Nyberg
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  3. Warren L. Grayson
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Corresponding author

Correspondence to Warren L. Grayson .

Editor information

Editors and Affiliations

  1. Ottawa Hospital Research Institute, Ottawa, ON, Canada

    Kursad Turksen

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© 2017 Springer Science+Business Media New York

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Rindone, A.N., Nyberg, E., Grayson, W.L. (2017). 3D-Printing Composite Polycaprolactone-Decellularized Bone Matrix Scaffolds for Bone Tissue Engineering Applications. In: Turksen, K. (eds) Decellularized Scaffolds and Organogenesis. Methods in Molecular Biology, vol 1577. Humana Press, New York, NY. https://doi.org/10.1007/7651_2017_37

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  • DOI: https://doi.org/10.1007/7651_2017_37

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7655-3

  • Online ISBN: 978-1-4939-7656-0

  • eBook Packages: Springer Protocols

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