Protocol

Part of the series Methods in Molecular Biology pp 1-18

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3D-Printing Composite Polycaprolactone-Decellularized Bone Matrix Scaffolds for Bone Tissue Engineering Applications

  • Alexandra N. RindoneAffiliated withTranslational TE Center, Johns Hopkins University School of MedicineDepartment of Biomedical Engineering, Johns Hopkins University School of Medicine 
  • , Ethan NybergAffiliated withTranslational TE Center, Johns Hopkins University School of MedicineDepartment of Biomedical Engineering, Johns Hopkins University School of Medicine 
  • , Warren L. GraysonAffiliated withTranslational TE Center, Johns Hopkins University School of MedicineDepartment of Biomedical Engineering, Johns Hopkins University School of MedicineDepartment of Materials Science and Engineering, Johns Hopkins UniversityInstitute for NanoBioTechnology, Johns Hopkins UniversityJohns Hopkins University, Translational Tissue Engineering Center Email author 

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Abstract

Millions of patients worldwide require bone grafts for treatment of large, critically sized bone defects from conditions such as trauma, cancer, and congenital defects. Tissue engineered (TE) bone grafts have the potential to provide a more effective treatment than current bone grafts since they would restore fully functional bone tissue in large defects. Most bone TE approaches involve a combination of stem cells with porous, biodegradable scaffolds that provide mechanical support and degrade gradually as bone tissue is regenerated by stem cells. 3D-printing is a key technique in bone TE that can be used to fabricate functionalized scaffolds with patient-specific geometry. Using 3D-printing, composite polycaprolactone (PCL) and decellularized bone matrix (DCB) scaffolds can be produced to have the desired mechanical properties, geometry, and osteoinductivity needed for a TE bone graft. This book chapter will describe the protocols for fabricating and characterizing 3D-printed PCL:DCB scaffolds. Moreover, procedures for culturing adipose-derived stem cells (ASCs) in these scaffolds in vitro will be described to demonstrate the osteoinductivity of the scaffolds.

Keywords

Bone tissue engineering 3D-printing Decellularized bone matrix Polycaprolactone Adipose-derived stem cells