Protocol

Part of the series Methods in Molecular Biology pp 1-10

Date:

Decellularization of Bovine Small Intestinal Submucosa

  • Mahmut ParmaksizAffiliated withTissue Engineering, Biomaterials and Nanobiotechnology Laboratory, Ankara University Faculty of Science, and Ankara University Stem Cell Institute
  • , Ayşe Eser ElçinAffiliated withTissue Engineering, Biomaterials and Nanobiotechnology Laboratory, Ankara University Faculty of Science, and Ankara University Stem Cell Institute
  • , Yaşar Murat ElçinAffiliated withBiovalda Health Technologies, Inc.Tissue Engineering, Biomaterials and Nanobiotechnology Laboratory, Ankara University Faculty of Science Email author 

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Abstract

Decellularization technology promises to overcome some of the significant limitations in the regenerative medicine field by providing functional biocompatible grafts. The technique involves removal of the cells from the biological tissues or organs for further use in tissue engineering and clinical interventions. There are significant differences between decellularization protocols due to the intrinsic properties of different tissue types and purpose of use. This multistep, chemical-solution-based protocol is optimized for the preparation of decellularized bovine small intestinal submucosa (SIS).

Keywords:

Decellularization Bovine Small intestinal submucosa (SIS) Xenogenic biomaterials Extracellular matrix (ECM) Regenerative medicine Bioactive materials