Protocol

Part of the series Methods in Molecular Biology pp 1-7

Date:

Preparation of Scaffolds from Decellularized Testicular Matrix

  • Yoni BaertAffiliated withBiology of the Testis, Research Laboratory for Reproduction, Genetics and Regenerative Medicine, Vrije Universiteit Brussel (VUB) Email author 
  • , Ellen GoossensAffiliated withBiology of the Testis, Research Laboratory for Reproduction, Genetics and Regenerative Medicine, Vrije Universiteit Brussel (VUB)

* Final gross prices may vary according to local VAT.

Get Access

Abstract

Biological scaffolds composed of extracellular matrix (ECM) are typically derived by processes that involve decellularization of tissues or organs. Here we describe a simple and robust methodology for the preparation of decellularized testicular matrix (DTM) scaffolds with minimal damage to the native three-dimensional structure and tissue-specific ECM components. Such DTM scaffolds can help to gain a better insight into the molecular composition and function of testicular ECM and to develop new tissue engineering approaches to treat various types of male fertility disorders.

Keywords:

Testis Extracellular matrix Decellularization Scaffold In vitro spermatogenesis