Protocol

Part of the series Methods in Molecular Biology pp 1-8

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A Protocol to Prepare Decellularized Stem Cell Matrix for Rejuvenation of Cell Expansion and Cartilage Regeneration

  • Jingting LiAffiliated withStem Cell and Tissue Engineering Laboratory, Department of Orthopaedics, Division of Exercise Physiology, and Mechanical and Aerospace Engineering, West Virginia University
  • , Ming PeiAffiliated withStem Cell and Tissue Engineering Laboratory, Department of Orthopaedics, West Virginia University Email author 

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Abstract

Traditional ex vivo expansion of adult stem cells yields an insufficient quantity of less potent cells. Here we describe the fabrication of decellularized matrix deposited by synovium-derived stem cells (SDSCs). This matrix could serve as a three-dimensional expansion system to rejuvenate cells for proliferation and tissue-specific differentiation potential, which could benefit cartilage regeneration. The decellularized stem cell matrix (DSCM) might be a powerful system for tissue engineering and regeneration.

Keywords

Decellularized stem cell matrix Cell expansion Synovium-derived stem cells Chondrogenesis Cartilage tissue engineering Cartilage regeneration