Protocol

Part of the series Methods in Molecular Biology pp 1-8

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Monitoring of Atg5-Independent Mitophagy

  • Satoko ArakawaAffiliated withDepartment of Pathological Cell Biology, Medical Research Institute, Tokyo Medical and Dental University
  • , Shinya HondaAffiliated withDepartment of Pathological Cell Biology, Medical Research Institute, Tokyo Medical and Dental University
  • , Satoru ToriiAffiliated withDepartment of Pathological Cell Biology, Medical Research Institute, Tokyo Medical and Dental University
  • , Masatsune TsujiokaAffiliated withDepartment of Pathological Cell Biology, Medical Research Institute, Tokyo Medical and Dental University
  • , Shigeomi ShimizuAffiliated withDepartment of Pathological Cell Biology, Medical Research Institute, Tokyo Medical and Dental University Email author 

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Abstract

Mitophagy is a mitochondrial quality control mechanism where damaged and surplus mitochondria are removed by autophagy. There are at least two different mammalian autophagy pathways: the Atg5-dependent conventional pathway and an Atg5-independent alternative pathway; the latter is involved in the erythrocyte mitophagy. In this chapter we describe the various experimental approaches to assess Atg5-indepedndent mitophagy in mammalian cells.

Keywords:

Mitophagy Atg5-independent autophagy Erythrocyte maturation Parkin Selective autophagy