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Microarray Data Analysis pp 1–10Cite as

Normalization of Affymetrix miRNA Microarrays for the Analysis of Cancer Samples

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1375))

Abstract

microRNA (miRNA) microarray normalization is a critical step for the identification of truly differentially expressed miRNAs. This is particularly important when dealing with cancer samples that have a global miRNA decrease. In this chapter, we provide a simple step-by-step procedure that can be used to normalize Affymetrix miRNA microarrays, relying on robust normal-exponential background correction with cyclic loess normalization.

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Acknowledgments

The authors thank Frances Cribbin for her help with the redaction of this review. The authors are supported by funding from the Australian NHMRC (1022144 and 1062683 to MPG and 1036541 to DW) and the Victorian Government’s Operational Infrastructure Support Program.

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Authors and Affiliations

  1. Department of Statistics, Harvard University, Cambridge, MA, USA

    Di Wu

  2. Centre for Cancer Research, MIMR-PHI Institute of Medical Research, 27-31 Wright Street, Clayton, VIC, 3168, Australia

    Di Wu & Michael P. Gantier

  3. Department of Molecular and Translational Science, Monash University, Clayton, VIC, Australia

    Michael P. Gantier

Authors
  1. Di Wu
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  2. Michael P. Gantier
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Corresponding author

Correspondence to Michael P. Gantier .

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Editors and Affiliations

  1. Department of Surgical and Medical Sciences, University “Magna Græcia” of Catanzaro, Catanzaro, Italy

    Pietro Hiram Guzzi

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Wu, D., Gantier, M.P. (2015). Normalization of Affymetrix miRNA Microarrays for the Analysis of Cancer Samples. In: Guzzi, P. (eds) Microarray Data Analysis. Methods in Molecular Biology, vol 1375. Humana Press, New York, NY. https://doi.org/10.1007/7651_2015_239

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  • DOI: https://doi.org/10.1007/7651_2015_239

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3172-9

  • Online ISBN: 978-1-4939-3173-6

  • eBook Packages: Springer Protocols

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