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Developmental Biology of the Sea Urchin and Other Marine Invertebrates

Volume 1128 of the series Methods in Molecular Biology pp 249-262

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Multicolor Labeling in Developmental Gene Regulatory Network Analysis

  • Aditya J. SethiAffiliated withNational Institute of Dental and Craniofacial Research, National Institutes of Health
  • , Robert C. AngererAffiliated withNational Institute of Dental and Craniofacial Research, National Institutes of Health
  • , Lynne M. AngererAffiliated withNational Institute of Dental and Craniofacial Research, National Institutes of Health

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Abstract

The sea urchin embryo is an important model system for developmental gene regulatory network (GRN) analysis. This chapter describes the use of multicolor fluorescent in situ hybridization (FISH) as well as a combination of FISH and immunohistochemistry in sea urchin embryonic GRN studies. The methods presented here can be applied to a variety of experimental settings where accurate spatial resolution of multiple gene products is required for constructing a developmental GRN.

Key words

TSA Plus Digoxigenin Fluorescein Lineage Signaling Linkages Marker Perturbations