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Molecular Plant Taxonomy

Volume 1115 of the series Methods in Molecular Biology pp 309-323

Date:

Molecular Cytogenetics (FISH and Fluorochrome Banding): Resolving Species Relationships and Genome Organization

  • Sonja Siljak-YakovlevAffiliated withLaboratory Ecology, Systematic and Evolution, UMR 8079, CNRS-UPS-AgroParisTech, University Paris-Sud
  • , Fatima PustahijaAffiliated withLaboratory Ecology, Systematic and Evolution, UMR 8079, CNRS-UPS-AgroParisTech, University Paris-SudFaculty of Forestry, University of Sarajevo
  • , Vedrana VicicAffiliated withDepartment of Molecular Biology, Faculty of Science, University of Zagreb
  • , Odile RobinAffiliated withLaboratory Ecology, Systematic and Evolution, UMR 8079, CNRS-UPS-AgroParisTech, University Paris-Sud

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Abstract

Fluorochrome banding (chromomycin, Hoechst, and DAPI) and fluorescence in situ hybridization (FISH) are excellent molecular cytogenetic tools providing various possibilities in the study of chromosomal evolution and genome organization. The constitutive heterochromatin and rRNA genes are the most widely used FISH markers. The rDNA is organized into two distinct gene families (18S-5.8S-26S and 5S) whose number and location vary within the complex of closely related species. Therefore, they are widely used as chromosomal landmarks to provide valuable evidence concerning genome evolution at chromosomal levels.

Key words

Chromomycin Crepis DAPI Fluorescence in situ hybridization (FISH) Fluorochrome banding Hoechst Pinus rRNA genes