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Gene Correction

Volume 1114 of the series Methods in Molecular Biology pp 143-161

Date:

Targeting piggyBac Transposon Integrations in the Human Genome

  • Daniel L. GalvanAffiliated withDepartment of Medicine, Baylor College of Medicine
  • , Claudia S. KettlunAffiliated withDepartment of Medicine, Baylor College of Medicine
  • , Matthew H. WilsonAffiliated withDepartment of Medicine and Center for Cell and Gene Therapy, Baylor College of Medicine and Michael E. DeBakey VA Medical Center

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Abstract

DNA based transposon systems offer a technology for active and efficient delivery of genes into human cells. An emerging field is directed at manipulating such systems to achieve site-directed integration as compared to un-targeted integration which occurs with native or unmodified transposon systems. The naturally active piggyBac transposon system is derived from insects but has been shown to be very efficient in gene-modifying human cells. Recent efforts have utilized the fusion of DNA binding domains to the piggyBac transposase enzyme with the goal of targeting integration to specific locations in the human genome. In this chapter, we describe methodology for engineering and characterizing chimeric piggyBac transposase enzymes, including experimental approaches for evaluating activity and targeting specificity in the human genome.

Key words

piggyBac Site-directed integration Transposon Transposase Zinc finger Human cells