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Environmental Microbiology

Volume 1096 of the series Methods in Molecular Biology pp 111-122

Date:

Analysis of Methanotroph Community Structure Using a pmoA-Based Microarray

  • Guy C. J. AbellAffiliated withCSIRO Marine and Atmospheric Research and Wealth from Ocean Flagship
  • , Nancy Stralis-PaveseAffiliated withDepartment of Bioresources, Austrian Institute of TechnologyDepartment of Biotechnology, University of Natural Resources and Applied Life Sciences
  • , Yao PanAffiliated withDepartment of Bioresources, Austrian Institute of Technology
  • , Levente BodrossyAffiliated withCSIRO Marine and Atmospheric Research and Wealth from Ocean FlagshipDepartment of Bioresources, Austrian Institute of Technology

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Abstract

The analysis of methanotroph community composition is relevant to studies of methane oxidation in a number of environments where methane is a significant carbon source. The development and application of a microarray targeting the particulate methane monooxygenase gene (pmoA) have allowed a high-throughput, semiquantitative analysis of the major methanotroph groups in a number of different environments.

Here we describe the use of a pmoA-based short oligo array for the analysis of methanotroph populations in sediment samples. The method is suitable for analysis of any type of environmental sample from which DNA can be extracted.

Key words

Microarray Methanotroph pmoA Hybridization