Protocol

Plant Metabolism

Volume 1083 of the series Methods in Molecular Biology pp 151-171

Date:

Putting the Plant Metabolic Network Pathway Databases to Work: Going Offline to Gain New Capabilities

  • Kate DreherAffiliated withCarnegie Institution for Science

* Final gross prices may vary according to local VAT.

Get Access

Abstract

Metabolic databases such as The Plant Metabolic Network/MetaCyc and KEGG PATHWAY are publicly accessible resources providing organism-specific information on reactions and metabolites. KEGG PATHWAY depicts metabolic networks as wired, electronic circuit-like maps, whereas the MetaCyc family of databases uses a canonical textbook-like representation. The first MetaCyc-based database for a plant species was AraCyc, which describes metabolism in the model plant Arabidopsis. This database was created over 10 years ago and has since then undergone extensive manual curation to reflect updated information on enzymes and pathways in Arabidopsis. This chapter describes accessing and using AraCyc and its underlying Pathway Tools software. Specifically, methods for (1) navigating Pathway Tools, (2) visualizing omics data and superimposing the data on a metabolic pathway map, and (3) creating pathways and pathway components are discussed.

Key words

Plant metabolism Omics data analysis Biochemical pathway databases