Protocol

Arabidopsis Protocols

Volume 1062 of the series Methods in Molecular Biology pp 53-62

Date:

Growing Arabidopsis In Vitro: Cell Suspensions, In Vitro Culture, and Regeneration

  • Bronwyn J. BarklaAffiliated withInstituto de Biotecnología, Universidad Nacional Autónoma de México
  • , Rosario Vera-EstrellaAffiliated withInstituto de Biotecnología, Universidad Nacional Autónoma de México
  • , Omar PantojaAffiliated withInstituto de Biotecnología, Universidad Nacional Autónoma de México

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Abstract

An understanding of basic methods in Arabidopsis tissue culture is beneficial for any laboratory working on this model plant. Tissue culture refers to the aseptic growth of cells, organs, or plants in a controlled environment, in which physical, nutrient, and hormonal conditions can all be easily manipulated and monitored. The methodology facilitates the production of a large number of plants that are genetically identical over a relatively short growth period. Techniques, including callus production, cell suspension cultures, and plant regeneration, are all indispensable tools for the study of cellular biochemical and molecular processes. Plant regeneration is a key technology for successful stable plant transformation, while cell suspension cultures can be exploited for metabolite profiling and mining. In this chapter we report methods for the successful and highly efficient in vitro regeneration of plants and production of stable cell suspension lines from leaf explants of both Arabidopsis thaliana and Arabidopsis halleri.

Key words

Callus Cell suspensions Plant regeneration Tissue culture Arabidopsis Organ regeneration